Tuesday, May 5, 2020

Microscope free essay sample

Lab Report Observing Bacteria and Blood Questions: List the following parts of the microscope and describe the function of each A. Ocular Lens- the lens on the top of the microscope that look into with 10x or 15x power. B. Body Tube- Connects eye piece to the objective lens C. Revolving Nosepiece- holds two or more objectives lenses and can be rotated easily to change power D. Objective lenses- 4x, 10x, 40x, and 100x powers E. Stage- The flat plate where the slides are placed for observation F. Diaphragm- Generally a five hold disc placed under the stage. Used to vary the intensity and size of the cone of light to see the slide. G. Illuminator- A light source, used to reflect light from an external light source up through the bottom of the stage. H. Coarse Focus Knob- Rough focus knob on the microscope used to move the objective lenses towards or away from the specimen. We will write a custom essay sample on Microscope or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page I. Fine Focus Knob- Knob used to fine tune the focus on the specimen, used after the coarse focus knob. J. Arm- Part of microscope that connects the tube to the base, used when carrying. K. Stage Clip- Clips on the stage used to hold the slide into place L. Base- bottom support of the microscope B. Define the following microscopy terms: a. Focus: Means of moving the specimen closer or further away from the objective lens and get a sharper image b. Resolution: Ability of a lens system to show details of the object c. Contrast: Darkness of the background relative to the specimen C. Describe your observations from fresh yogurt slide you prepared in Part III. We did not do the fresh yogurt slide. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, describe them. The prepared yogurt slide was purple spheres and commas covering the whole slide. E. Describe the four main bacterial shapes The four main bacterial shapes were spheres, rods, commas, and spirals. F. What are the common arrangements bacteria are found in? Bacteria are found in pairs (diplo), strands (strepto), or clusters (staphlo). G. Were you able to identify specific bacterial morphologies on either yogurt slide? If so, which types? On the prepared yogurt slide I was able to identify spheres and commas. H. Describe the cells you were able to see in the blood smear. I was able to see platelets and white blood cells in the blood smear. I. Are the cells you observed in your blood smear different than the bacterial cells you have observed? Why or why not? Yes they are different. They have similar shape but the bacterial cells were smaller while the blood smear was a lot more going on. J. What is the purpose of immersion oil? Why does it work? The purpose is to allow a larger picture of the tiny things on the slide. Oil is placed on the slide to prevent the loss of light due to light rays passing through the air. It enhances the resolving power of the microscope.

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